1EIF

EUKARYOTIC TRANSLATION INITIATION FACTOR 5A FROM METHANOCOCCUS JANNASCHII

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	1997-11
Detector	MARRESEARCH
Spacegroup name	P 41 2 2
Unit cell lengths	45.520, 45.520, 155.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.205
Rwork	0.205
R-free	0.28900
Structure solution method	MIR, MAD
RMSD bond length	0.012
RMSD bond angle	30.030 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.85)
Refinement software	X-PLOR (3.85)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.039	0.326
Number of reflections	12622
<I/σ(I)>	24.1	5.3
Completeness [%]	92.3	97.7
Redundancy	4.02	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.6		0.1 M SODIUM ACETATE (PH 4.6) 8% PEK4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium acetate	0.1 (M)
3	1	drop	PEG4000	8 (%(w/v))