1EIA

X-RAY CRYSTAL STRUCTURE OF EQUINE INFECTIOUS ANEMIA VIRUS (EIAV) CAPSID PROTEIN P26

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1997-11-17
Detector	BRANDEIS
Spacegroup name	I 4 3 2
Unit cell lengths	176.760, 176.760, 176.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 2.700
R-factor	0.227
Rwork	0.227
R-free	0.27500
Structure solution method	TREAT MAD AS MIR
RMSD bond length	0.009
RMSD bond angle	18.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.086	0.357 *
Number of reflections	12292 *
<I/σ(I)>	40	6
Completeness [%]	99.4	94.6
Redundancy		17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	22 (mg/ml)
2	1	drop	sodium phosphate	1 (mM)
3	1	reservoir	sodium citrate	0.1 (M)
4	1	reservoir	PEG3350	10 (%(w/v))
5	1	reservoir	isopropanol	15 (%(v/v))