1EHL

64M-2 ANTIBODY FAB COMPLEXED WITH D(5HT)(6-4)T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-07-26
Detector	RIGAKU
Spacegroup name	C 1 2 1
Unit cell lengths	144.500, 48.062, 77.939
Unit cell angles	90.00, 104.69, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.199
Rwork	0.192
R-free	0.27900
RMSD bond length	0.014
RMSD bond angle	1.871
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.700	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.084	0.221
Total number of observations	77929 *
Number of reflections	18790	1571 *
<I/σ(I)>	18.5
Completeness [%]	93.5	79.1
Redundancy	4.1	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	PEG 8000, nickel chloride, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	64M-2 Fab	0.2 (mM)
2	1	drop	dT(6-4)T	0.25 (mM)
3	1	reservoir	PEG8000	14-18 (%(w/v))
4	1	reservoir	HEPES-Na	0.1 (M)	or Tris-HCl, pH7.5-8.4
5	1	reservoir		10 (mM)