1EH4
BINARY COMPLEX OF CASEIN KINASE-1 FROM S. POMBE WITH AN ATP COMPETITIVE INHIBITOR, IC261
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 133 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-26 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 113.500, 113.500, 110.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.30400 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.000 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.109 | |
| Total number of observations | 98304 * | |
| Number of reflections | 18625 | |
| Completeness [%] | 95.5 | 76.4 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | 16 * | Ammonium Sulfate, Sodium Acetate, MPD , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | MOPS | 10 (mM) | |
| 3 | 1 | drop | EGTA | 0.1 (mM) | |
| 4 | 1 | drop | 2-mercaptoethanol | 0.1 (%) | |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | 2 (%(v/v)) | ||
| 7 | 1 | reservoir | ammonium sulfate | 1.5-1.6 (M) | |
| 8 | 1 | reservoir | sodium acetate | 5 (mM) | |
| 9 | 1 | reservoir | MPD | 1 (%) |
