1EGK
CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-20 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 62.810, 114.260, 171.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.100 |
R-factor | 0.2296 |
Rwork | 0.226 |
R-free | 0.25800 |
RMSD bond length | 0.011 |
RMSD bond angle | 28.900 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 3.100 |
Rmerge | 0.051 |
Number of reflections | 10948 |
<I/σ(I)> | 7.1 |
Completeness [%] | 99.2 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 297.5 | 50MM SODIUM CACODYLATE 6.0, 20MM MAGNESIUM CHLORIDE, 1MM SPERMINE, 25% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 24.5K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SODIUM CACODYLATE | ||
2 | 1 | 1 | SPERMINE | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
2 | 1 | reservoir | 20 (mM) | ||
3 | 1 | reservoir | spermine | 1 (mM) | |
4 | 1 | reservoir | 25 (%(v/v)) |