1EFW
Crystal structure of aspartyl-tRNA synthetase from Thermus thermophilus complexed to tRNAasp from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 123 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 63 |
Unit cell lengths | 251.450, 251.450, 88.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 3.000 |
R-factor | 0.246 * |
Rwork | 0.248 |
R-free | 0.29100 * |
RMSD bond length | 0.010 |
RMSD bond angle | 1.820 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.088 | 0.239 |
Number of reflections | 58634 | |
<I/σ(I)> | 14.9 | |
Completeness [%] | 91.9 | 98.3 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 17 * | sodium citrate, magnesium chloride, Na-HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | sodium citrate | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | Na-HEPES | ||
4 | 1 | 2 | sodium citrate | ||
5 | 1 | 2 | MgCl2 | ||
6 | 1 | 2 | Na-HEPES |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | tRNA | 7.35 (mg/ml) | |
3 | 1 | reservoir | sodium citrate | 0.7 (M) | |
4 | 1 | reservoir | 10 (mM) | ||
5 | 1 | reservoir | Na-HEPES | 50 (mM) |