1EFT

THE CRYSTAL STRUCTURE OF ELONGATION FACTOR EF-TU FROM THERMUS AQUATICUS IN THE GTP CONFORMATION

Experimental procedure

Source type	ROTATING ANODE
Spacegroup name	C 1 2 1
Unit cell lengths	150.700, 99.700, 40.200
Unit cell angles	90.00, 95.30, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.2
Rwork	0.200
RMSD bond length	0.012
RMSD bond angle	1.300
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.200 *	2.200 *
Rmerge		0.450 *
Number of reflections		1443 *
Completeness [%]		74.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.7 *	19 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	32 (mM)
2	1	drop		0.5 (mM)
3	1	drop	dithiothreitol	0.5 (mM)
4	1	drop	GDPNP	0.3 (mM)
5	1	drop	AS	18-22 (%)
6	1	drop	EF-Tu.GDPNP	2.5-5 (mg/ml)
7	1	reservoir	Tris-HCl	32 (mM)
8	1	reservoir		0.5 (mM)
9	1	reservoir	AS	45-47