1EFO
CRYSTAL STRUCTURE OF AN ADENINE BULGE IN THE RNA CHAIN OF A DNA/RNA HYBRID, D(CTCCTCTTC)/R(GAAGAGAGAG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-22 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.660, 47.610, 54.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.22200 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.069 * | 0.080 |
| Number of reflections | 2578 * | |
| Completeness [%] | 91.4 | 71.3 |
| Redundancy | 5.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | MPD, LITHIUM CHLORIDE, SPERMINE TETRACHLORIDE, CACODYLATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SPERMINE TETRACHLORIDE | ||
| 2 | 1 | 1 | CACODYLATE | ||
| 3 | 1 | 1 | LICL | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | LICL | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mM) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | spermine tetrachloride | 10 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 5 | 1 | drop | MPD | 5 (%(v/v)) | |
| 6 | 1 | reservoir | 40 (%) |
