1EFH
CRYSTAL STRUCTURE OF THE HUMAN HYDROXYSTEROID SULFOTRANSFERASE IN THE PRESENCE OF PAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 1999-08-27 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.190, 96.820, 127.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.23900 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | 0.146 |
| Total number of observations | 113997 * | |
| Number of reflections | 34110 * | |
| <I/σ(I)> | 15.3 | |
| Completeness [%] | 94.4 | 86.9 |
| Redundancy | 3.34 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 * | 300 | 0.8 M citrate, 80 mM cacodylate, 4 mM PAP, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 25 (mg/ml) | |
| 2 | 1 | 2 | HEPES | 25 (mM) | |
| 3 | 1 | 2 | PAP | 4 (mM) | |
| 4 | 1 | 2 | citrate | 0.8 (M) | |
| 5 | 1 | 2 | cacodylate | 80 (mM) |
