1EF7
CRYSTAL STRUCTURE OF HUMAN CATHEPSIN X
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-14 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.251, 92.480, 209.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.670 |
| R-factor | 0.183 |
| R-free | 0.22600 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | MAIN |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.400 | 2.830 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.062 | 0.240 |
| Total number of observations | 152861 * | |
| Number of reflections | 16732 | |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 95.0 | 87 * |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 4000, 2-propanol, Na-HEPES, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.8 (mg/ml) | |
| 2 | 1 | drop | Na acetate | 20 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 14.4 (%) | |
| 5 | 1 | reservoir | 2-propanol | 7.2 (%) | |
| 6 | 1 | reservoir | Na-HEPES | 0.072 (M) |
