1EEQ

M4L/Y(27D)D/T94H Mutant of LEN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1998-09-21
Detector	CUSTOM-MADE
Spacegroup name	P 21 21 2
Unit cell lengths	62.500, 105.200, 42.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.990 - 1.500
R-factor	0.211
Rwork	0.211
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1LVE. Dimer generated by crystallographic operation. Atoms beyond CB removed for mutated residues.
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.3C)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.049	0.207
Number of reflections	44489
<I/σ(I)>	46.6	6.6
Completeness [%]	98.8	95.6
Redundancy	17	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20% PEG 3350, 10% 2-propanol, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	20 (%)
2	1	reservoir	2-propanol	10 (%)
3	1	reservoir	sodium HEPES	0.1 (M)
4	1	drop	protein	10 (mg/ml)