1EDM
EPIDERMAL GROWTH FACTOR-LIKE DOMAIN FROM HUMAN FACTOR IX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-01-01 |
| Detector | SIEMENS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 40.300, 40.300, 98.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.157 |
| Rwork | 0.157 |
| Structure solution method | MIR |
| Starting model (for MR) | NMR MODEL |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | 3D-SCALE ((D. STUART)) |
| Data scaling software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.650 |
| High resolution limit [Å] | 1.500 * | 1.500 |
| Rmerge | 0.102 * | |
| Total number of observations | 48451 * | |
| Number of reflections | 13041 * | |
| Completeness [%] | 94.5 * | 92 |
| Redundancy | 3.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.3 | 289 * | pH 7.3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | calcium chloride | 20 (mM) | |
| 4 | 1 | reservoir | PEG400 | 48 (%) |
