1ECY
PROTEASE INHIBITOR ECOTIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-06-24 |
| Detector | FUJI |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.320, 63.320, 84.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.190 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.31900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ECOTIN PART OF ECOTIN-TRYPSIN COMPLEX |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.670 * |
| Data reduction software | WEIS |
| Data scaling software | WEIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.000 | 2.290 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.073 | |
| Number of reflections | 8537 | |
| Completeness [%] | 91.6 | 73.2 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | 22.5-23.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | ADA | 50 (mM) | |
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | drop | PEG1500 | 20 (%(w/v)) | |
| 5 | 1 | drop | trehalose | 0.1 (%(w/v)) | |
| 6 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 7 | 1 | reservoir | PEG1500 | 36 (%(w/v)) |
