1ECJ
ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 AMP PER TETRAMER
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 279 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-02 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 116.800, 156.000, 106.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gph |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.320 * |
| Data reduction software | WEIS |
| Data scaling software | Agrovata |
| Phasing software | GLRF |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.065 * | |
| Total number of observations | 199847 * | |
| Number of reflections | 55291 | |
| <I/σ(I)> | 37 | 4.3 |
| Completeness [%] | 77.8 | 56.1 |
| Redundancy | 3.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.3 | 27 * | 100MM MES, 50MM TRIS, 2MM AMP, 4MM EDTA, 15-18% PEG-3350, PH 6.3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 100 (mM) | |
| 10 | 1 | drop | PEG3350 | 7.5-9 (%(w/v)) | |
| 11 | 1 | drop | protein | 3.5-17.5 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 50 (mM) | |
| 3 | 1 | reservoir | AMP | 2 (mM) | |
| 4 | 1 | reservoir | EDTA | 4 (mM) | |
| 5 | 1 | reservoir | PEG3350 | 15-18 (%(w/v)) | |
| 6 | 1 | drop | MES | 50 (mM) | |
| 7 | 1 | drop | Tris | 25 (mM) | |
| 8 | 1 | drop | AMP | 1 (mM) | |
| 9 | 1 | drop | EDTA | 2 (mM) |
