1EC7
E. COLI GLUCARATE DEHYDRATASE NATIVE ENZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1998-02-11 |
| Detector | OTHER |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.250, 84.550, 98.920 |
| Unit cell angles | 103.30, 94.00, 113.10 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.194 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.700 | 1.900 |
| Rmerge | 0.070 | 0.202 |
| Total number of observations | 526735 * | |
| Number of reflections | 150262 * | |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 95.2 | 90 |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 | 277 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG5000 ME | 14 (%) | |
| 2 | 1 | 1 | 75 (mM) | ||
| 3 | 1 | 1 | 2-propanol | 5 (%) | |
| 4 | 1 | 1 | HEPPES | 50 (mM) |
