1EC6
CRYSTAL STRUCTURE OF NOVA-2 KH3 K-HOMOLOGY RNA-BINDING DOMAIN BOUND TO 20-MER RNA HAIRPIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-09-01 |
| Detector | OTHER |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.850, 51.210, 128.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.208 * |
| Rwork | 0.208 |
| R-free | 0.29100 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.081 | 0.211 * |
| Total number of observations | 143241 * | |
| Number of reflections | 67678 * | |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 98.0 * | 93.7 |
| Redundancy | 5.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | 20 * | protein:RNA 1:1.25 ratio * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | (NH4)2SO4 | ||
| 3 | 1 | 1 | PEG 5000 | ||
| 4 | 1 | 2 | (NH4)2SO4 | ||
| 5 | 1 | 2 | PEG 5000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG5000 MME | 25 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 150 (mM) | |
| 3 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
| 4 | 1 | drop | protein | 0.155 (mM) |
