1EBK
Structural and kinetic analysis of drug resistant mutants of HIV-1 protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.410, 61.950, 51.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.060 |
| Rwork | 0.192 |
| R-free | 0.30600 |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.990 * |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.084 |
| Number of reflections | 19007 |
| Completeness [%] | 79.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 298 | CITRATE/PHOSPHATE BUFFER 0.05M, DTT 10MM, DMSO 10%, SATURATED AMMONIUM SULPHATE (25-50%), PROTEIN 2-5 MG/ML, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protease | 4.5-8.0 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 20-55 (mM) | |
| 3 | 1 | reservoir | sodium citrate | 66 (mM) | |
| 4 | 1 | reservoir | sodium phosphate | 132 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 30-36 (%sat) |
