1EBA

COMPLEX BETWEEN THE EXTRACELLULAR DOMAIN OF ERYTHROPOIETIN (EPO) RECEPTOR [EBP] AND AN INACTIVE PEPTIDE [EMP33] CONTAINS 3,5-DIBROMOTYROSINE IN POSITION 4 (DENOTED DBY)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 2 21 21
Unit cell lengths	58.890, 75.009, 130.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.2
Rwork	0.200
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1EBP MONOMER
RMSD bond length	0.008
RMSD bond angle	26.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE ((CCP4))
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.500	2.700
Rmerge	0.089 *	0.300 *
Number of reflections	16100 *
<I/σ(I)>	19.2	4.1
Completeness [%]	99.0	99
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *	22 *	pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	mPEG5000	12 (%)
2	1	reservoir	sodium cacodylate	0.100 (mM)