1EAX

Crystal structure of MTSP1 (matriptase)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-08-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 2
Unit cell lengths	66.918, 141.604, 51.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 * - 1.300
R-factor	0.184
Rwork	0.184
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ekb
RMSD bond length	0.010
RMSD bond angle	25.800 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000	1.360
High resolution limit [Å]	1.300	1.300
Rmerge	0.086	0.294
Number of reflections	58805
<I/σ(I)>	4	1.3
Completeness [%]	96.2	93.5
Redundancy	3.3	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8	18 *	pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)	pH8.0
2	1	reservoir	ammonium sulfate	1.5 (M)
3	1	reservoir	ethanol	3 (%)