1EAQ
The RUNX1 Runt domain at 1.25A resolution: A structural switch and specifically bound chloride ions modulate DNA binding
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-06 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9315,0.9793,0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.300, 46.400, 63.000 |
| Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
| Resolution | 37.000 * - 1.250 |
| R-factor | 0.149 |
| Rwork | 0.149 |
| R-free | 0.17100 * |
| Structure solution method | MAD |
| RMSD bond length | 0.021 * |
| RMSD bond angle | 2.150 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.060 | 0.245 |
| Total number of observations | 606863 * | |
| Number of reflections | 71531 | |
| <I/σ(I)> | 18.3 | 2 |
| Completeness [%] | 97.7 | 78 |
| Redundancy | 8.48 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 25 % PEG 3350, 16% GLYCEROL, 130 MM NA CACODYLATE, PH 6.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 1.6 (M) | ||
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | HEPES | 20 (mM) | pH8.0 |
| 4 | 1 | drop | protein | 23 (mg/ml) | |
| 5 | 1 | reservoir | PEG3350 | 25 (%(w/v)) | |
| 6 | 1 | reservoir | glycerol | 16 (%(v/v)) | |
| 7 | 1 | reservoir | sodium cacodylate | 130 (mM) | pH6.4 |
