1E9R
Bacterial conjugative coupling protein TrwBdeltaN70. Trigonal form in complex with sulphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 151.300, 151.300, 258.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.393 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 72.600 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.057 |
| Number of reflections | 1205165 |
| <I/σ(I)> | 9 |
| Completeness [%] | 100.0 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 1.5 M AMMONIUM SULPHATE, 0.1 M NACL, 0.1 M HEPES PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium sulfate | 1.5 (M) | |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | drop | HEPES | 0.1 (M) |
