1E8V
Structure of the multifunctional paramyxovirus hemagglutinin-neuraminidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Spacegroup name | P 61 |
Unit cell lengths | 137.460, 137.460, 116.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.380 |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.800 | |
Rmerge | 0.052 | 0.262 * |
Total number of observations | 498619 * | |
Number of reflections | 38673 | |
Completeness [%] | 94.0 | 98 * |
Redundancy | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.2 * | pH 6.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | sialic acid | 10 (mM) | |
3 | 1 | reservoir | PEG3350 | 20 (%) | |
4 | 1 | reservoir | HEPES | 0.1 (M) |