1E8S
Alu domain of the mammalian SRP (potential Alu retroposition intermediate)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-11-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.7757,1.7753,1.033,0.9326 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 143.330, 143.330, 60.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.460 - 4.000 |
R-factor | 0.388 |
Rwork | 0.388 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 22.100 * |
Data reduction software | HKL |
Data scaling software | HKL |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.100 |
High resolution limit [Å] | 4.000 | 4.000 |
Number of reflections | 5053 | |
<I/σ(I)> | 5.9 | 1.5 |
Completeness [%] | 90.3 | 59.6 |
Redundancy | 2.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 12 * | 50MM HEPES, 10MM MGCL2, 150MM NACL, 0.8 MM EU(NO3)3, 390MM (NH4)2SO4, 23% PEG400, pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | SA88 RNP | 0.070 (mM) | |
10 | 1 | reservoir | 10 (mM) | pH7.0 | |
11 | 1 | reservoir | 150 (mM) | ||
12 | 1 | reservoir | Eu(NO3)3 | 0.80 (mM) | |
13 | 1 | reservoir | ammonium sulfate | 390 (mM) | |
14 | 1 | reservoir | PEG400 | 23 (%) | |
2 | 1 | drop | HEPES | 28 (mM) | pH7.0 |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | 75 (mM) | ||
5 | 1 | drop | Eu(NO3)3 | 0.40 (mM) | |
6 | 1 | drop | dithiothreitol | 5 (mM) | |
7 | 1 | drop | ammonium sulfate | 210 (mM) | |
8 | 1 | drop | PEG400 | 12 (%) | |
9 | 1 | reservoir | HEPES | 50 (mM) |