1E7S
GDP 4-keto-6-deoxy-D-mannose epimerase reductase K140R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 103.400, 103.400, 75.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 1.500 |
| Rwork | 0.126 |
| R-free | 0.16200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bws |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.067 | |
| Number of reflections | 73654 | |
| <I/σ(I)> | 8.65 | 2.1 |
| Completeness [%] | 98.8 | 97.7 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 21 * | 1.5 M LITHIUM SULPHATE, PH 6.5 0.1M TRIS BUFFER 21C |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 11-20 (mg/ml) | |
| 2 | 1 | 2 | lithium sulfate | 1.5 (M) | |
| 3 | 1 | 2 | MES | 0.1 (M) | orTris |
