1E7L

Endonuclease VII (EndoVII) N62D mutant from phage T4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-15
Detector	MAR scanner 345 mm plate
Spacegroup name	P 1 21 1
Unit cell lengths	58.240, 35.830, 91.930
Unit cell angles	90.00, 103.93, 90.00

Refinement procedure

Resolution	35.000 - 1.320
R-factor	0.145
Rwork	0.146
R-free	0.18600
Structure solution method	MIRAS
RMSD bond length	0.010
RMSD bond angle	0.019
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	1.370
High resolution limit [Å]	1.320	1.320
Rmerge	0.075 *	0.301 *
Total number of observations	243182 *
Number of reflections	81068
<I/σ(I)>	9.9	2.3
Completeness [%]	96.5	88.9
Redundancy	2.99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	4 *	HANGING DROP VAPOUR DIFFUSION DROP SIZE: 1 + 1 UL PROTEIN SOLUTION: 12 MG/ML ENDOVII, 175 MM NACL, 20 MM MGCL2, 2 MM ZNCL2, 10 MM 2-MERCAPTO-ETHANOL, 10 % GLYCEROL, 10 MM MOPS PH6 WELL: 16-18% PEG5KMME, 200 MM AMMONIUM SULPHATE, 10 MM 2-MERCAPTO-ETHANOL, 100 MM TRIS PH 4.5, ~1 MM SODIUM AZIDE, 20 MM MAGNESIUM ACETATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	PEG5000 MME	15.5-16 (%(w/v))
3	1	reservoir	sodium acetate	100 (mM)
4	1	reservoir	ammonium sulfate	160 (mM)
5	1	reservoir	magnesium acetate	20 (mM)
6	1	reservoir	beta-mercaptoethanol	10 (mM)