1E7K
Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Collection date | 1999-03-15 |
| Wavelength(s) | 0.9184, 0.9790, 0.9791, 0.9919 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.756, 55.287, 146.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.30200 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.210 |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.055 * | 0.118 * |
| Total number of observations | 28438 * | |
| Number of reflections | 8125 | |
| Completeness [%] | 93.2 | 89.3 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 | 21 * | pH 7.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | HEPES-NaOH | 0.1 (M) | |
| 3 | 1 | reservoir | PEG2000 | 16.5 (%) | |
| 4 | 1 | reservoir | 30 (mM) | ||
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 6 | 1 | reservoir | sodium azide | 0.04 (%(w/v)) |
