1E6U
GDP 4-keto-6-deoxy-D-mannose epimerase reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 102.800, 102.800, 74.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.450 |
Rwork | 0.135 |
R-free | 0.16700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bws |
RMSD bond length | 0.015 |
RMSD bond angle | 0.032 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.057 | |
Number of reflections | 80994 | |
<I/σ(I)> | 14.8 | 5.5 |
Completeness [%] | 99.1 | 99 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 | 21 * | 1.5 M LITHIUM SULPHATE, PH 6.5 0.1M TRIS BUFFER 21C |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 11-20 (mg/ml) | |
2 | 1 | 2 | lithium sulfate | 1.5 (M) | |
3 | 1 | 2 | MES | 0.1 (M) | or Tris |