1E3O
Crystal structure of Oct-1 POU dimer bound to MORE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.300, 52.400, 69.000 |
| Unit cell angles | 90.00, 127.60, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oct |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.039 | 0.111 |
| Number of reflections | 91810 | |
| <I/σ(I)> | 24.4 | 6.2 |
| Completeness [%] | 95.3 | 97 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 20 * | Remenyi, A., (2001) Acta Crystallogr., D57, 1634. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 22-24 (%(v/w)) | |
| 2 | 1 | reservoir | HEPES | 50 (mM) | |
| 3 | 1 | reservoir | spermine | 1.8 (mM) |
