1E31
SURVIVIN DIMER H. SAPIENS
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.27557, 1.28243, 1.28283, 1.60478 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 82.220, 71.050, 89.450 |
| Unit cell angles | 90.00, 96.12, 90.00 |
Refinement procedure
| Resolution | 27.760 - 2.710 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.27000 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 21.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.064 | 0.229 |
| Number of reflections | 12978 | |
| <I/σ(I)> | 9.66 | 2.51 |
| Completeness [%] | 92.8 | 75.9 |
| Redundancy | 2.46 * | 1.77 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | 3 % PEG MME 2K, 2 MM HEXAMINE COBALT, 100 MM HEPES PH 7.1 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | ||
| 2 | 1 | reservoir | PEG2000 MME | 4 (%) | |
| 3 | 1 | reservoir | ethylene glycol | 5 (%) | |
| 4 | 1 | reservoir | hexamine cobalt chloride | 1 (mM) |
