1E06

Porcine Odorant Binding Protein Complexed with 5-methyl-2-(1-methylethyl)phenol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-12-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	42.076, 88.287, 93.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.120
R-factor	0.213
Rwork	0.213
R-free	0.25700 *
Structure solution method	OTHER
RMSD bond length	0.008
RMSD bond angle	27.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000	2.150
High resolution limit [Å]	2.120	2.120
Rmerge	0.029 *	0.168 *
Number of reflections	23430
<I/σ(I)>	15	5.1
Completeness [%]	99.0	99.3
Redundancy	3.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.8	20 *	2M AMMONIUM SULFATE, AND 5% ISOPROPANOL, pH 7.80

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	iso-propanol	5 (%(v/v))