1DZO

Truncated PAK pilin from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-13
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-12-15
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	38.114, 38.114, 149.775
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.440 - 1.630
Rwork	0.153
R-free	0.18100
Structure solution method	MIRAS
RMSD bond length	0.012
RMSD bond angle	0.023
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	ARP/wARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.440	1.720
High resolution limit [Å]	1.633	1.630
Rmerge	0.049 *	0.190 *
Total number of observations	110628 *
Number of reflections	14500 *
<I/σ(I)>	26.7	10.1
Completeness [%]	99.5	96.4
Redundancy	7.6	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.2		HANGING DROP USING 1 ML OF RESERVOIR DROPS MADE FROM 3 MICROLITRE PROTEIN AND 3 MICROLITRE OF MOTHER LIQUOR PROTEIN SOLUTION = 10 MG/ML IN WATER MOTHER LIQUOR = 60% (NH4)2SO4, 0.1M HEPES PH 8.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	ammonium sulfate	60 (%)
3	1	1	HEPES	0.1 (M)
4	1	2	ammonium sulfate	60 (%)
5	1	2	HEPES	0.1 (M)