1DZN

Asp170Ser mutant of vanillyl-alcohol oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	1999-02-15
Detector	MARRESEARCH
Spacegroup name	I 4
Unit cell lengths	131.330, 131.330, 134.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.22686
Rwork	0.227
R-free	0.29130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qlt
RMSD bond length	0.013
RMSD bond angle	0.043
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.128	0.323 *
Total number of observations	23315 *
Number of reflections	16730
<I/σ(I)>	4.7	4.6
Completeness [%]	83.7	83.8 *
Redundancy	2.4	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		pH 4.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	potassium phosphate	50 (mM)
3	1	reservoir	sodium acetate/hydrochloride	50 (mM)
4	1	reservoir	PEG4000	5 (%(w/v))