1DZM

Porcine Odorant Binding Protein Complexed with benzoic acid phenylmethylester

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-09-24
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	42.009, 88.679, 93.163
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.930
R-factor	0.235
Rwork	0.235
R-free	0.25600 *
Structure solution method	OTHER
RMSD bond length	0.015
RMSD bond angle	1.660 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.053 *	0.258 *
Number of reflections	26679
<I/σ(I)>	13.7	3.3
Completeness [%]	99.2	99.9
Redundancy	3.35 *	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.8	20 *	PROTEIN (0.47MM) IS INCUBATED WITH 10MM OF PYRAZINE, THE COMPLEX WAS CRYSTALLISED FROM 2M AMMONIUM SULFATE, AND 5% ISOPROPANOL IN HANGING DROPS, AT 20 DEGREES, pH 7.80

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	iso-propanol	5 (%(v/v))