1DYR

THE STRUCTURE OF PNEUMOCYSTIS CARINII DIHYDROFOLATE REDUCTASE TO 1.9 ANGSTROMS RESOLUTION

Experimental procedure

Collection date	1992-06-24
Detector	SIEMENS
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	69.900, 43.600, 37.600
Unit cell angles	90.00, 117.70, 90.00

Refinement procedure

Resolution	10.000 - 1.860
R-factor	0.181
RMSD bond length	0.016
RMSD bond angle	0.033
Data reduction software	XENGEN
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.050
Total number of observations	41714 *
Number of reflections	15602	1193 *
Completeness [%]	83.0	39 *
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6 *	4 *	Stammers, D.K., (1993) J.Mol.Biol., 230, 679. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3400	15-19 (%(w/v))
2	1	reservoir	MES	50 (mM)