1DYO

Xylan-Binding Domain from CBM 22, formally x6b domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-10-15
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	89.490, 89.490, 207.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.19
Rwork	0.190
R-free	0.25000
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	0.036
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.120	2.120
Rmerge	0.074	0.437
Number of reflections	141380
<I/σ(I)>	19.2	3.4
Completeness [%]	98.0	96.5
Redundancy	5.11	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6		CRYSTALS WERE GROWN FROM A PROTEIN CONCENTRATION OF 50 MG/ML IN 0.1 M NAAC BUFFER, PH 4.6, CONTAINING 10 MM DTT, 25 % (V/V) GLYCEROL AND WITH 12 % (W/V) PEG 8000 AS THE PRECIPITANT

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	drop		0.1 (mg/ml)
3	1	drop	dithiothreitol	10 (mM)
4	1	drop	glycerol	25 (%(v/v))
5	1	drop	PEG8000	12 (%(w/v))