1DYM
Humicola insolens Endocellulase Cel7B (EG 1) E197A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.969, 74.284, 60.146 |
| Unit cell angles | 90.00, 103.82, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| Rwork | 0.158 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2A39 NATIVE STRUCTURE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.076 | 0.450 |
| Number of reflections | 43135 | |
| <I/σ(I)> | 19.7 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20-30% PEG 4K, 20 MM TRIS-HCL PH 8.0 |
