1DXY
STRUCTURE OF D-2-HYDROXYISOCAPROATE DEHYDROGENASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-08-01 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 134.100, 134.100, 125.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.100 - 1.860 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BACKBONE AND C-BETA ATOMS OF D-GLYCERATE DEHYDROGENASE (PDB ENTRY 1GDH) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 23.120 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.100 | 2.000 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.089 * | 0.480 * |
| Total number of observations | 314570 * | |
| Number of reflections | 53838 | |
| <I/σ(I)> | 22.2 | 2.8 |
| Completeness [%] | 96.5 | 86.9 |
| Redundancy | 5.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 4 * | Niefind, K., (1994) J.Mol.Biol., 240, 400. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium salfate | 1.8 (M) | |
| 2 | 1 | reservoir | potassium phosphate | 50 (mM) | |
| 3 | 1 | drop | ammonium salfate | 0.9 (M) | |
| 4 | 1 | drop | potassium phosphate | 25 (mM) | |
| 5 | 1 | drop | protain | 5 (mg/ml) | |
| 6 | 1 | drop | potassium phosphate | 25 (mM) | |
| 7 | 1 | drop | dithiothreitol | 0.5 (mM) |
