1DXH
Catabolic ornithine carbamoyltransferase from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Detector | BRUKER NONIUS |
Spacegroup name | I 2 3 |
Unit cell lengths | 134.320, 134.320, 134.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.179 |
Rwork | 0.179 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ort |
RMSD bond length | 0.014 |
RMSD bond angle | 1.500 |
Data reduction software | MADNESS |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.610 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.075 * | 0.258 * |
Total number of observations | 80597 * | |
Number of reflections | 13231 | |
Completeness [%] | 95.4 | 65.5 * |
Redundancy | 5.58 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.2 | pH 7.20 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 1.85 (M) | |
3 | 1 | reservoir | HEPES | 50 (mM) | pH7.2 |
4 | 1 | reservoir | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | EDTA | 1 (mM) | |
6 | 1 | reservoir | PEG400 | 2 (%) | |
7 | 1 | reservoir | spermidine | 10 (mM) |