1DVP

CRYSTAL STRUCTURE OF THE VHS AND FYVE TANDEM DOMAINS OF HRS, A PROTEIN INVOLVED IN MEMBRANE TRAFFICKING AND SIGNAL TRANSDUCTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	1999-07-08
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	116.706, 69.665, 41.799
Unit cell angles	90.00, 94.77, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.219
Rwork	0.215
R-free	0.25400
RMSD bond length	0.016
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.069	0.290
Total number of observations	89859 *
Number of reflections	22957 *
<I/σ(I)>	15
Completeness [%]	99.8	100
Redundancy	3.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5 *	4 *	PEG10000, Sodium Citrate, Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	dithiothreitol	1 (mM)
3	1	drop	citrate	50 (mM)
4	1	reservoir	PEG10000	15 (%)
5	1	reservoir	dithiothreitol	5 (mM)
6	1	reservoir	HEPES	100 (mM)