1DVP
CRYSTAL STRUCTURE OF THE VHS AND FYVE TANDEM DOMAINS OF HRS, A PROTEIN INVOLVED IN MEMBRANE TRAFFICKING AND SIGNAL TRANSDUCTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-07-08 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.706, 69.665, 41.799 |
| Unit cell angles | 90.00, 94.77, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.219 |
| Rwork | 0.215 |
| R-free | 0.25400 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.290 |
| Total number of observations | 89859 * | |
| Number of reflections | 22957 * | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 * | 4 * | PEG10000, Sodium Citrate, Hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | citrate | 50 (mM) | |
| 4 | 1 | reservoir | PEG10000 | 15 (%) | |
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) |
