1DVK

CRYSTAL STRUCTURE OF THE FUNCTIONAL DOMAIN OF THE SPLICING FACTOR PRP18

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-10-10
Detector	FUJI
Spacegroup name	C 2 2 21
Unit cell lengths	49.935, 79.167, 171.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.150
R-factor	0.203
Rwork	0.203
R-free	0.25200
RMSD bond length	0.005
RMSD bond angle	0.985
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.041	0.097
Total number of observations	188477 *
Number of reflections	17664
<I/σ(I)>	16.9
Completeness [%]	93.2	55.7
Redundancy	10.7	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	17 *	100mM MES 6.0, 25% PEG8000, 10% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	MES	100 (mM)	pH6.0
3	1	reservoir	glycerol	10 (%)
4	1	reservoir	PEG8000	20 (%)