1DVK
CRYSTAL STRUCTURE OF THE FUNCTIONAL DOMAIN OF THE SPLICING FACTOR PRP18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-10 |
Detector | FUJI |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.935, 79.167, 171.191 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.150 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.25200 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.985 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.041 | 0.097 |
Total number of observations | 188477 * | |
Number of reflections | 17664 | |
<I/σ(I)> | 16.9 | |
Completeness [%] | 93.2 | 55.7 |
Redundancy | 10.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 17 * | 100mM MES 6.0, 25% PEG8000, 10% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-10 (mg/ml) | |
2 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
3 | 1 | reservoir | glycerol | 10 (%) | |
4 | 1 | reservoir | PEG8000 | 20 (%) |