1DUZ

HUMAN CLASS I HISTOCOMPATIBILITY ANTIGEN (HLA-A 0201) IN COMPLEX WITH A NONAMERIC PEPTIDE FROM HTLV-1 TAX PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-13
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-11-27
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	50.560, 63.790, 75.080
Unit cell angles	81.58, 75.66, 77.38

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.1977 *
Rwork	0.198
R-free	0.25020
RMSD bond length	0.030
RMSD bond angle	2.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Rmerge	0.042 *
Total number of observations	137168 *
Number of reflections	72184 *
Completeness [%]	85.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	13-20% polyethylene glycol 6000, 25 mM MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	MES	25 (mM)
3	1	reservoir	PEG6000	13-20 (%)
4	1	reservoir	MES	25 (mM)