1DUH
CRYSTAL STRUCTURE OF THE CONSERVED DOMAIN IV OF E. COLI 4.5S RNA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-08-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0302, 1.3366, 1.3369, 0.9968, 1.3359 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 69.697, 69.697, 84.102 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.500 - 2.700 |
R-factor | 0.244 |
Rwork | 0.230 |
R-free | 0.24500 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.500 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.076 | 0.593 |
Total number of observations | 154595 * | |
Number of reflections | 12371 | |
<I/σ(I)> | 14.5 | 2.1 |
Completeness [%] | 98.5 | 100 |
Redundancy | 4.2 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 303.15 | CRYSTALLIZED FROM 1.60-1.90 M (NH4)2SO4, 0.09 M MAGNESIUM ACETATE, 0.05 M SODIUM CACODYLATE PH 6.0, AT 303 K. CRYSTALS WERE STABILISED AT 292 K IN A SOLUTION OF 2.20 M (NH4)2SO4, 0.01 M MGCL2, 0.05 M BIS-TRIS-HCL PH 6.0. SOAK CONDITIONS: CRYSTALS WERE SOAKED IN STABILISATION SOLUTION CONTAINING 0.002 M LUTETIUM CHLORIDE HEXAHYDRATE. CRYOPROTECTION CONDITIONS: AFTER ADDITION OF 20% GLYCEROL (W/V) TO THE SOAK SOLUTION, CRYSTALS WERE FLASH-FROZEN IN LIQUID NITROGEN., VAPOR DIFFUSION, SITTING DROP, temperature 303.15K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MAGNESIUM ACETATE | ||
2 | 1 | 1 | SODIUM CACODYLATE | ||
3 | 1 | 1 | BIS-TRIS-HCL | ||
4 | 1 | 1 | (NH4)2SO4 | ||
5 | 1 | 1 | MGCL2 | ||
6 | 1 | 1 | LUCL3 | ||
7 | 1 | 2 | (NH4)2SO4 | ||
8 | 1 | 2 | BIS-TRIS-HCL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.6-1.8 (M) | |
2 | 1 | reservoir | 90 (mM) | ||
3 | 1 | reservoir | Na cacodylate | 50 (mM) | |
4 | 1 | reservoir | 2 (mM) |