1DUC
EIAV DUTPASE DUDP/STRONTIUM COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 106.700, 106.700, 106.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.050 |
| R-factor | 0.1686 * |
| Rwork | 0.167 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dup |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.040 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.124 | 0.686 |
| Total number of observations | 142838 * | |
| Number of reflections | 13598 | |
| <I/σ(I)> | 17.7 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.5 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 4 * | DROP: 3.0 MG/ML PROTEIN, 0.05 M IMIDAZOL MALATE BUFFER, PH 7.0, 21% PEG 400, 20 MM SRCL2, 5 MM DUDP; WELL: 0.1 M IMIDAZOLE MALATE BUFFER, PH 7.0, 42% PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.5-1.6 (mg/ml) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | dUDP | 5 (mM) | |
| 4 | 1 | reservoir | PEG400 | 21 (%(v/v)) |
