1DT4

CRYSTAL STRUCTURE OF NOVA-1 KH3 K-HOMOLOGY RNA-BINDING DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1998-03-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 42 21 2
Unit cell lengths	44.670, 44.670, 84.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.245 *
Rwork	0.222
R-free	0.31000 *
RMSD bond length	0.014 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.041	0.190
Number of reflections	2473
<I/σ(I)>	24.3
Completeness [%]	91.2	90.3
Redundancy	4.3	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	20 *	Li2SO4, sodium acetate, sodium cloride, pH 4.0, VAPOR DIFFUSION, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		1.8 (M)
2	1	reservoir		100 (mM)
3	1	drop	protein	10 (mg/ml)
4	1	drop		300 (mM)
5	1	drop	HEPES	20 (mM)