1DSX
KV1.2 T1 DOMAIN, RESIDUES 33-119, T46V MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 1999-08-20 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.390, 78.398, 126.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.234 * |
| Rwork | 0.237 |
| R-free | 0.27900 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.750 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.048 | 0.371 |
| Number of reflections | 97159 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 96.1 * | 84.4 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 | 18 * | 22% PEG 1500, 5 % ISOPROPANOL, 200 MM NA ACETATE, 12 MM SRCL2, 50 MM TRIS, PH 8.5, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG1500 | 22 (%) | |
| 3 | 1 | reservoir | isopropanol | 5 (%) | |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | 12 (mM) | ||
| 6 | 1 | reservoir | Tris-HCl | 50 (mM) |
