1DRU

ESCHERICHIA COLI DHPR/NADH COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1995-09-25
Detector	SIEMENS
Spacegroup name	I 2 2 2
Unit cell lengths	81.200, 84.500, 91.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.192
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DHPR-NADPH
RMSD bond length	0.019
RMSD bond angle	20.000 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	AMoRE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.076 *
Number of reflections	19014
<I/σ(I)>	9.1	1.5
Completeness [%]	77.1	39.3
Redundancy	3.7	1.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		Scapin, G., (1995) Biochemistry, 34, 3502. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	2.2 (M)
2	1	reservoir	HEPES	100 (mM)	pH7.5
3	1	drop	protein	18 (mg/ml)
4	1	drop	ammonium salfate	2.2 (M)
5	1	drop	HEPES	100 (mM)
6	1	drop	NADPH	1.5-2.0 (M)