1DQX

CRYSTAL STRUCTURE OF OROTIDINE 5'-PHOSPHATE DECARBOXYLASE COMPLEXED TO 6-HYDROXYURIDINE 5'-PHOSPHATE (BMP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1999-06-05
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	79.890, 79.970, 98.190
Unit cell angles	90.00, 108.59, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.213
Rwork	0.213
R-free	0.25800
RMSD bond length	0.015
RMSD bond angle	1.900
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.061	0.122
Number of reflections	42013
<I/σ(I)>	11.1
Completeness [%]	91.3	78.4
Redundancy	6.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	22 *	sodium phosphate, polyethylene glycol 3350, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium phosphate	0.18 (M)
2	1	reservoir	PEG3350	18 (%(w/v))