1DPY
THREE-DIMENSIONAL STRUCTURE OF A NOVEL PHOSPHOLIPASE A2 FROM INDIAN COMMON KRAIT AT 2.45 A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1999-09-14 |
Detector | MARRESEARCH |
Spacegroup name | R 3 2 |
Unit cell lengths | 57.980, 57.980, 57.980 |
Unit cell angles | 92.02, 92.02, 92.02 |
Refinement procedure
Resolution | 20.000 * - 2.400* |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.27100 * |
RMSD bond length | 0.007 |
RMSD bond angle | 22.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.600 |
High resolution limit [Å] | 2.400 * | 2.400 * |
Rmerge | 0.092 * | 0.174 * |
Number of reflections | 4629 | |
<I/σ(I)> | 7.2 | |
Completeness [%] | 93.0 * | 92 * |
Redundancy | 17.2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 8 * | NACL, TRIS HCL, EDTA, NAN3, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 281K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | drop | 1.4 (M) | ||
4 | 1 | drop | 1 (mM) | ||
5 | 1 | reservoir | Tris-HCl | 50 (mM) | |
6 | 1 | reservoir | 3.5 (M) |