1DPO
STRUCTURE OF RAT TRYPSIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | AREA DETECTOR |
| Collection date | 1988-12 |
| Detector | SIEMENS |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 123.120, 123.120, 123.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.590 |
| R-factor | 0.174 * |
| Rwork | 0.174 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 0.049 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.105 * | |
| Number of reflections | 41613 | |
| <I/σ(I)> | 27.2 | 4 |
| Completeness [%] | 97.0 | 81.6 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | magnesium sulfate | 55 (%) | |
| 2 | 1 | reservoir | calcium chloride | 10 (mM) | |
| 3 | 1 | reservoir | benzamidine | 75-100 (mM) | |
| 4 | 1 | reservoir | Tris | 50 (mM) | |
| 5 | 1 | drop | protain | 30 (mg/ml) |
