1DOK

MONOCYTE CHEMOATTRACTANT PROTEIN 1, P-FORM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-11-05
Detector	RIGAKU
Spacegroup name	P 43 21 2
Unit cell lengths	50.740, 50.740, 107.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.850
R-factor	0.191
Rwork	0.191
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dom
RMSD bond length	0.014
RMSD bond angle	27.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.078 *	0.363 *
Number of reflections	12318
<I/σ(I)>	15.3	2.15
Completeness [%]	97.0	94.7
Redundancy	4.59	3.82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8		10 MG/ML PROTEIN IN 50 MM TRIS BUFFER PH 7.5-8 EQUILIBRATED AGAINST 50-55% AMMONIUM SULFATE USING HANGING DROP VAPOR DIFFUSION METHOD., pH 8.0, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	reservoir	ammonium sulfate	50-55 (%(w/v))